Approaches to Full/Empty Quantification: Implementation of Methods for Early-Stage Programs
Time: 1:30 pm
day: Workshop H
Details:
- SEC, AUC, cryo-EM, ddPCR/ELISA, Stunner, and SEC-MALS were assessed for the quantification of program-specific rAAV
- Chromatography-based methods were easily adaptable for routine testing of partially to fully purified materials
- AUC was effective with the quantification of partial genomes
- SEC/MALS and FFF/MALS for comprehensive characterization of AAV vector-based gene therapy products
