Approaches to Full/Empty Quantification: Implementation of Methods for Early-Stage Programs

Time: 1:30 pm
day: Workshop H


  • SEC, AUC, cryo-EM, ddPCR/ELISA, Stunner, and SEC-MALS were assessed for the quantification of program-specific rAAV
  • Chromatography-based methods were easily adaptable for routine testing of partially to fully purified materials
  • AUC was effective with the quantification of partial genomes
  • SEC/MALS and FFF/MALS for comprehensive characterization of AAV vector-based gene therapy products