Evaluating current and future LC and LCMS technologies for the analysis of rAAV’s with the goal to minimize sample demand

Time: 2:40 pm
day: Track C - Day 2 PM


  • LC and LCMS for the characterization of AAV capsid proteins at the peptide level with reduced sample consumption.
  • Measurement of VP expression with fluorescence detection and intact mass/post-translational modifications (PTMs) analysis using a benchtop Time-of-Flight (ToF) mass spectrometer
  • Anion exchange chromatography and CDMS analysis of AAV ssDNA content
  • Size-Exclusion Chromatography (SEC) analysis of dimeric and other low valency HMW forms
  • High-throughput SEC for AAV titer and ssDNA content